Rnf130^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnf130^{tm1a(EUCOMM)Wtsi}
• Name: ring finger protein 130; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432601
• Synonyms: Rnf130^-, Rnf130^KO1-Tm1a
• Gene: Rnf130 Location: Chr11:49916173-50016546 bp, + strand Genetic Position: Chr11, 30.16 cM, cytoband B1.2
• Alliance: Rnf130^{tm1a(EUCOMM)Wtsi} page
• IMPC: Rnf130 gene page

Mutation origin

• Mutant Cell Lines: EPD0237_1_A08, EPD0237_1_B06, EPD0237_1_C07, EPD0237_1_G05, EPD0237_1_H06
• Germline Transmission: Earliest citation of germline transmission: J:338210
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 49961273 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 49962754. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Rnf130 Mutation: 26 strains or lines available

Notes

Special note about the mutation generated in J:338210: authors state that mice homozygous for the Rnf130^{tm1a(EUCOMM)Wtsi} allele exhibit 30% residual Rnf130 mRNA expression in the liver and brain, indicating a hypomorphic mutation. Furthermore, heterozygotes only show a 30% reduction in mRNA expression (versus expected 50%). None of the commercially available antibodies tested were found to be specific for the endogenous mouse RNF130.

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)