Mecr^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mecr^{tm1a(EUCOMM)Wtsi}
• Name: mitochondrial trans-2-enoyl-CoA reductase; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432643
• Synonyms: Mecr^tm1a
• Gene: Mecr Location: Chr4:131570781-131595097 bp, + strand Genetic Position: Chr4, 64.52 cM
• Alliance: Mecr^{tm1a(EUCOMM)Wtsi} page
• IMPC: Mecr gene page

Mutation origin

• Mutant Cell Lines: EPD0039_5_A01, EPD0039_5_B02, EPD0039_5_B03, EPD0039_5_C01, EPD0039_5_C02, EPD0039_5_D02, EPD0039_5_G01, EPD0039_5_H02, EPD0039_5_H03
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 131580788 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 131581364. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:242847)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Mecr Mutation: 17 strains or lines available

Notes

Special note about mutant cell line EPD0039_5_G01: The mutation generated in this ES cell line results in a leaky allele, with homozygous embryos displaying embryonic lethality over a broad window of time and due to a variety of causes. A clear lipoylated Dlat signal was observed in protein extracts from homozygous embryos at E11.5, in line with the proposed allele leakiness, allowing for synthesis of low levels of lipoic acid (LA) used for modification of Dlat (J:242847).

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)