Sec23b^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sec23b^{tm1a(EUCOMM)Wtsi}
• Name: SEC23 homolog B, COPII coat complex component; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432981
• Synonyms: Sec23b^cgt, Sec23b^gt2
• Gene: Sec23b Location: Chr2:144398165-144432673 bp, + strand Genetic Position: Chr2, 71.02 cM, cytoband H1
• Alliance: Sec23b^{tm1a(EUCOMM)Wtsi} page
• IMPC: Sec23b gene page

Degeneration of pancreas, salivary, gastric and nasal glands in Sec23b^{tm1a(EUCOMM)Wtsi}/Sec23b^{tm1a(EUCOMM)Wtsi} mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0237_3_C05, EPD0237_3_C08, EPD0237_3_D08, EPD0237_3_E08, EPD0237_3_G06
• Germline Transmission: Earliest citation of germline transmission: J:186391
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_Pgk_P Mutation details: The L1L2_Pgk_P cassette was inserted at position 144408239 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 144409359. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Schematic of the Sec23b^tm1a(EUCOMM)Wtsi allele

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Sec23b Mutation: 50 strains or lines available

Notes

Cell line EPD0237_3_G06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:186391

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 7 reference(s)