Pik3c3^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pik3c3^{tm1a(EUCOMM)Wtsi}
• Name: phosphatidylinositol 3-kinase catalytic subunit type 3; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4433098
• Gene: Pik3c3 Location: Chr18:30405800-30481179 bp, + strand Genetic Position: Chr18, 17.41 cM
• Alliance: Pik3c3^{tm1a(EUCOMM)Wtsi} page
• IMPC: Pik3c3 gene page

Mutation origin

• Mutant Cell Lines: EPD0103_4_A07, EPD0103_4_B07, EPD0103_4_B08, EPD0103_4_D07, EPD0103_4_D09, EPD0103_4_E08, EPD0103_4_F09, EPD0103_4_G08, EPD0103_4_G09, EPD0103_4_H09
• Germline Transmission: Earliest citation of germline transmission: J:165965
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_gt2 Mutation details: The L1L2_gt2 cassette was inserted at position 30414548 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 30415389. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Generation of the Pik3c3^tm1a(EUCOMM)Wtsi and Pik3c3^tm1c(EUCOMM)Wtsi alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Pik3c3 Mutation: 48 strains or lines available

Notes

Cell line EPD0103_4_A07 was used to make chimeric mice. Germline transmission was accomplished. J:182628

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)