Pdzd7^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pdzd7^{tm1a(EUCOMM)Wtsi}
• Name: PDZ domain containing 7; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4433578
• Gene: Pdzd7 Location: Chr19:45015345-45034156 bp, - strand Genetic Position: Chr19, 38.23 cM
• Alliance: Pdzd7^{tm1a(EUCOMM)Wtsi} page
• IMPC: Pdzd7 gene page

Mutation origin

• Mutant Cell Lines: EPD0237_5_C06, EPD0237_5_D07, EPD0237_5_E05, EPD0237_5_E07
• Germline Transmission: Earliest citation of germline transmission: J:209073
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 45027360 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 45029623. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Pdzd7 Mutation: 49 strains or lines available

Notes

Digenic heterozygous mice of Pdzd7 with Ush2a, Gpr98, Whrn or Ush1g exhibit normal ABR thresholds at P24. J:209073

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)