Kptn^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kptn^{tm1a(EUCOMM)Wtsi}
• Name: kaptin; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4433701
• Synonyms: Kptn^-, Kptn KO, Kptn^tm1a
• Gene: Kptn Location: Chr7:15853820-15861441 bp, + strand Genetic Position: Chr7, 8.75 cM
• Alliance: Kptn^{tm1a(EUCOMM)Wtsi} page
• IMPC: Kptn gene page

Mutation origin

• Mutant Cell Lines: EPD0183_4_A10, EPD0183_4_A12, EPD0183_4_C10, EPD0183_4_D10, EPD0183_4_E10, EPD0183_4_E11, EPD0183_4_G10
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 15856463 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 15858298. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. RNA-Seq read counts for Kptn expression showed an 87-90% reduction in E18 brain and adult brain (hippocampus, cortex, cerebellum) samples from homozygous mutant mice, indicating that this is a loos-of-function allele leading to nonsense-mediated decay of Kptn mRNA in vivo. (J:155845, J:173534, J:343200)

Disease models

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Kptn Mutation: 20 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 6 reference(s)