Cyfip2^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyfip2^{tm1a(EUCOMM)Wtsi}
• Name: cytoplasmic FMR1 interacting protein 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4434196
• Gene: Cyfip2 Location: Chr11:46084677-46203686 bp, - strand Genetic Position: Chr11, 27.61 cM, cytoband B1.2
• Alliance: Cyfip2^{tm1a(EUCOMM)Wtsi} page
• IMPC: Cyfip2 gene page

Mutation origin

• Mutant Cell Lines: EPD0436_2_A04, EPD0436_2_B03, EPD0436_2_H02
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 46170548 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 46171429. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Mice Carrying this Mutation:	22 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Cyfip2 Mutation: 61 strains or lines available

Notes

Cell lines EPD0436_2_H02 and EPD0436_2_A04 were successfully used to make chimeric mice. Germline transmission was accomplished for both lines. Cell line EPD0436_2_B03 was successfully used to make chimeric mice but germline transmission was not achieved. J:203183

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 14 reference(s)