Ube3b^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ube3b^{tm1a(EUCOMM)Hmgu}
• Name: ubiquitin protein ligase E3B; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4434627
• Gene: Ube3b Location: Chr5:114518668-114559230 bp, + strand Genetic Position: Chr5, 55.99 cM
• Alliance: Ube3b^{tm1a(EUCOMM)Hmgu} page
• IMPC: Ube3b gene page

Growth retardation in Ube3b^{tm1a(EUCOMM)Hmgu}/Ube3b^{tm1a(EUCOMM)Hmgu} mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: HEPD0074_4_A09, HEPD0074_4_B07, HEPD0074_4_B09, HEPD0074_4_C07, HEPD0074_4_C08, HEPD0074_4_C09, HEPD0074_4_D07, HEPD0074_4_D09, HEPD0074_4_E07, HEPD0074_4_E08, HEPD0074_4_E09, HEPD0074_4_F07, HEPD0074_4_F08, HEPD0074_4_F09, HEPD0074_4_G07, HEPD0074_4_G08, HEPD0074_4_G09, HEPD0074_4_H07, HEPD0074_4_H08, HEPD0074_4_H09
• Germline Transmission: Earliest citation of germline transmission: J:273879
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_gt0 Mutation details: The L1L2_gt0 cassette was inserted at position 114527986 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 114528771. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:273879)

Schematic of the Ube3b^tm1a(EUCOMM)Hmgu allele

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Ube3b Mutation: 70 strains or lines available

Notes

Cell line HEPD0074_4_C07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:273879.

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)