Usp20^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Usp20^{tm1a(EUCOMM)Hmgu}
• Name: ubiquitin specific peptidase 20; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4434938
• Gene: Usp20 Location: Chr2:30872291-30912667 bp, + strand Genetic Position: Chr2, 21.77 cM, cytoband B
• Alliance: Usp20^{tm1a(EUCOMM)Hmgu} page
• IMPC: Usp20 gene page

Mutation origin

• Mutant Cell Lines: HEPD0524_2_A10, HEPD0524_2_A11, HEPD0524_2_B09, HEPD0524_2_B10, HEPD0524_2_B11, HEPD0524_2_B12, HEPD0524_2_C09, HEPD0524_2_C10, HEPD0524_2_C11, HEPD0524_2_C12, HEPD0524_2_D09, HEPD0524_2_D10, HEPD0524_2_D11, HEPD0524_2_E10, HEPD0524_2_E12, HEPD0524_2_F09, HEPD0524_2_F11, HEPD0524_2_F12, HEPD0524_2_G09, HEPD0524_2_H09, HEPD0524_2_H10, HEPD0524_2_H11
• Germline Transmission: Earliest citation of germline transmission: J:277075
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30893994 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 30895294. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Usp20 Mutation: 51 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 4 reference(s)