Peg3^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Peg3^{tm1a(EUCOMM)Hmgu}
• Name: paternally expressed 3; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435596
• Synonyms: Peg3^CoKO
• Gene: Peg3 Location: Chr7:6706891-6733430 bp, - strand Genetic Position: Chr7, 3.89 cM, cytoband A2-B1
• Alliance: Peg3^{tm1a(EUCOMM)Hmgu} page
• IMPC: Peg3 gene page

Mutation origin

• Mutant Cell Line: HEPD0535_3_C12
• Germline Transmission: Earliest citation of germline transmission: J:209833
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 6718777 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 6719585. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:209833)

Expression

In Mice Carrying this Mutation:	129 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Peg3 Mutation: 61 strains or lines available

Notes

Mutant cell line HEPD0535_3_C12 was used in J:209833 to generate a mouse line.

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 9 reference(s)