Aktip^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Aktip^{tm1a(EUCOMM)Hmgu}
• Name: AKT interacting protein; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435660
• Synonyms: Ft1^kof
• Gene: Aktip Location: Chr8:91834267-91862122 bp, - strand Genetic Position: Chr8, 44.25 cM
• Alliance: Aktip^{tm1a(EUCOMM)Hmgu} page
• IMPC: Aktip gene page

Mutation origin

• Mutant Cell Lines: HEPD0589_6_D06, HEPD0589_6_H06, HEPD0636_4_A09, HEPD0636_4_B09, HEPD0636_4_B10, HEPD0636_4_B11, HEPD0636_4_C12, HEPD0636_4_D11, HEPD0636_4_E09, HEPD0636_4_E11, HEPD0636_4_F09, HEPD0636_4_G10, HEPD0636_4_H09, HEPD0636_4_H12
• Germline Transmission: Earliest citation of germline transmission: J:276082
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 91853169 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 91854047. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Expression

In Structures Affected by this Mutation:

14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Aktip Mutation: 24 strains or lines available

Notes

Cell line HEPD0589_6_H06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:276082

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 4 reference(s)