Smug1^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Smug1^{tm1a(EUCOMM)Hmgu}
• Name: single-strand selective monofunctional uracil DNA glycosylase; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435952
• Gene: Smug1 Location: Chr15:103061717-103075519 bp, - strand Genetic Position: Chr15, 58.2 cM
• Alliance: Smug1^{tm1a(EUCOMM)Hmgu} page
• IMPC: Smug1 gene page

Mutation origin

• Mutant Cell Lines: HEPD0529_1_A08, HEPD0529_1_B07, HEPD0529_1_B08, HEPD0529_1_C05, HEPD0529_1_F08
• Germline Transmission: Earliest citation of germline transmission: J:197809
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 103061483 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 103065065. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Expression

In Mice Carrying this Mutation:

7 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Smug1 Mutation: 14 strains or lines available

Notes

Cell line HEPD0529_1_C05 and HEPD0529_1_B07 were successfully used to make chimeric mice. Germline transmission was accomplished. J:197809

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 3 reference(s)