Katnb1^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Katnb1^{tm1a(EUCOMM)Hmgu}
• Name: katanin p80 (WD40-containing) subunit B 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4436305
• Gene: Katnb1 Location: Chr8:95807804-95826502 bp, + strand Genetic Position: Chr8, 47.12 cM, cytoband C5
• Alliance: Katnb1^{tm1a(EUCOMM)Hmgu} page
• IMPC: Katnb1 gene page

Mutation origin

• Mutant Cell Lines: HEPD0589_5_B02, HEPD0589_5_D03, HEPD0589_5_F02, HEPD0636_3_A09, HEPD0636_3_A10, HEPD0636_3_B09, HEPD0636_3_B11, HEPD0636_3_C09, HEPD0636_3_C11, HEPD0636_3_D09, HEPD0636_3_D11, HEPD0636_3_E11, HEPD0636_3_F09, HEPD0636_3_F10, HEPD0636_3_G09, HEPD0636_3_G10, HEPD0636_3_G12, HEPD0636_3_H10
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 95816164 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 95816925. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Expression

In Mice Carrying this Mutation:	41 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Katnb1 Mutation: 37 strains or lines available

Notes

Cell line HEPD0636_3_F10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:220901

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 5 reference(s)