Wdr35^{tm2a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Wdr35^{tm2a(EUCOMM)Hmgu}
• Name: WD repeat domain 35; targeted mutation 2a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4436424
• Gene: Wdr35 Location: Chr12:9023897-9078848 bp, + strand Genetic Position: Chr12, 3.97 cM, cytoband A1.3
• Alliance: Wdr35^{tm2a(EUCOMM)Hmgu} page
• IMPC: Wdr35 gene page

Mutation origin

• Mutant Cell Lines: HEPD0518_6_A11, HEPD0518_6_B09, HEPD0518_6_B11, HEPD0518_6_C11, HEPD0518_6_D09, HEPD0518_6_D11, HEPD0518_6_E09, HEPD0518_6_F09, HEPD0518_6_F10, HEPD0518_6_F12, HEPD0518_6_G10
• Germline Transmission: Earliest citation of germline transmission: J:171617
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 9024468 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 9025271. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Wdr35 Mutation: 70 strains or lines available

Notes

HEPD0518_6_E09 and HEPD0518_6_F10 ES cells were used to generate the mice used in J:171617.

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)