Foxp3^tm8Ayr
Targeted Allele Detail

Summary

• Symbol: Foxp3^tm8Ayr
• Name: forkhead box P3; targeted mutation 8, Alexander Y Rudensky
• MGI ID: MGI:4436759
• Synonyms: Foxp3^gfpko
• Gene: Foxp3 Location: ChrX:7445915-7461482 bp, + strand Genetic Position: ChrX, 3.41 cM
• Alliance: Foxp3^tm8Ayr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157377
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The complete EGFP coding sequence was inserted in-frame in the first coding exon followed immediately by a cassette containing three stop codons. This same stop codon cassette was also inserted in exon 5 near its 5' end. An FRT site flanked neomycin resistance gene cassette was inserted 246 bp 5' of exon 1 as well. Chimeric mice were subsequently mated to an flp-deleter strain to excise the neo selection cassette. The full length endogenous protein is not expressed. (J:157377)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxp3 Mutation: 58 strains or lines available

References

• Original: J:157377 Gavin MA, et al., Foxp3-dependent programme of regulatory T-cell differentiation. Nature. 2007 Feb 15;445(7129):771-5
• All: 10 reference(s)