H2-Ab1^b-tm1.1Sish
Targeted Allele Detail

Summary

• Symbol: H2-Ab1^b-tm1.1Sish
• Name: histocompatibility 2, class II antigen A, beta 1; targeted mutation 1.1, Satoshi Ishido
• MGI ID: MGI:4436864
• Synonyms: H2-Ab1^tm1.1Sish, I-Abeta^K225R, MHC-II I-A^b K225R-knockin
• Gene: H2-Ab1 Location: Chr17:34482201-34488392 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: H2-Ab1^b-tm1.1Sish page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157516
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP was inserted upstream of exon 3 and a STOP cassette followed by a floxed neo cassette and modified genomic sequence for exon 3 through 6. The modified exon 4 contains a nucleotide substitution that results in the amino acid substitution of arginine for lysine at position 225 (K225R). This amino acid is responsible for the ubiquitination-mediated degradation of the peptide. Cre mediated recombination removes the wild-type exon 3 through 6 and the neo cassette allowing for expression of a transcript containing the nucleotide substitution. (J:157516)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any H2-Ab1 Mutation: 83 strains or lines available

References

• Original: J:157516 Ohmura-Hoshino M, et al., Cutting edge: requirement of MARCH-I-mediated MHC II ubiquitination for the maintenance of conventional dendritic cells. J Immunol. 2009 Dec 1;183(11):6893-7
• All: 7 reference(s)