Rest^tm1.1Yasu
Targeted Allele Detail

Summary

• Symbol: Rest^tm1.1Yasu
• Name: RE1-silencing transcription factor; targeted mutation 1.1, Yasuhiro Yamada
• MGI ID: MGI:4437069
• Synonyms: Rest^2lox, Rest^floxed
• Gene: Rest Location: Chr5:77413338-77434279 bp, + strand Genetic Position: Chr5, 41.61 cM
• Alliance: Rest^tm1.1Yasu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157076
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exon 4 was replaced with a construct containing the following elements: loxP site, exon 4, IRES, GFP, loxP, neo cassette, and loxP site. Cre mediated recombination removed the neo cassette leaving exon 4 following by an IRES sequence and GFP gene floxed. (J:157076)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rest Mutation: 96 strains or lines available

References

• Original: J:157076 Yamada Y, et al., Rest promotes the early differentiation of mouse ESCs but is not required for their maintenance. Cell Stem Cell. 2010 Jan 8;6(1):10-5
• All: 8 reference(s)