Gt(ROSA)26Sor^{tm1.1(CMV-luc,-ALPP)Cklr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CMV-luc,-ALPP)Cklr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Charles Keller
• MGI ID: MGI:4437208
• Synonyms: LuSEAP, Rosa26^LUSEAPm
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CMV-luc,-ALPP)Cklr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157754
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A dual-modality bioreporter was targeted to the Rosa26 locus in intron 1. In addition to the native Rosa26 promoter, the CMV immediate-early promoter/enhancer and the SV40 late viral protein gene 16S/19S splice donor and acceptor (sd/sa) signal sites were added to strengthen reporter gene activity. To minimize leakage, a stop cassette consisting of 6 tandem copies of the SV40 viral early and late polyadenylation signal sequences [(pA)6] were inserted downstream of the promoter elements, flanked by LoxP sites. The stop cassette is followed by firefly luciferase, a human IRES, and human placental secreted alkaline phosphatase. An FRT-flanked neo selection followed the hPLAP gene and was removed by breeding to FLPe transgenic mice. (J:157754)

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm1.1(CMV-luc,-ALPP)Cklr}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:157754 Nishijo K, et al., Biomarker system for studying muscle, stem cells, and cancer in vivo. FASEB J. 2009 Aug;23(8):2681-90
• All: 5 reference(s)