Fgf8^tm2.1Jyhl
Targeted Allele Detail

Summary

• Symbol: Fgf8^tm2.1Jyhl
• Name: fibroblast growth factor 8; targeted mutation 2.1, James Y H Li
• MGI ID: MGI:4437219
• Synonyms: Fgf8^deltaa
• Gene: Fgf8 Location: Chr19:45725237-45731354 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband C3-D
• Alliance: Fgf8^tm2.1Jyhl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156717
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A point mutation into the 3' alternative splice acceptor of Fgf8 exon 1D, changing the sequence from cagCATGTG to caaCATGTG. A floxed neo selection cassette was place 400 bp downstream of exon 1D. The cassette was removed by cre mediated recombination. RT-PCR demonstrated that the transcripts of Fgf8a-containing spliceforms (a, c, e, and g) were missing, while Fgf8b-containing spliceforms, which utilize the remaining alternative splice acceptor of the exon 1D, are expressed. (J:156717)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf8 Mutation: 21 strains or lines available

References

• Original: J:156717 Guo Q, et al., Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum. Dev Biol. 2010 Feb 15;338(2):183-92
• All: 1 reference(s)