Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo
Transgene Detail

Summary

• Symbol: Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo
• Name: transgene insertion 1, Andrew Wolfe
• MGI ID: MGI:4437221
• Synonyms: GnRH-CRETeR
• Transgene: Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo Location: unknown
• Alliance: Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Recombinase)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gnrh1 (MGI:95789)

• Mutation details: A -3446 bp fragment of the mouse Gnrh1 promoter was fused upstream of cre recombinase. The proximal region of the Gnrh1 promoter was modified to insert two Tet operators (tetO2). In series, the transgene also contains the CMV promoter regulating the Tet repressor gene (tetR). The rabbit beta-globin intron II is inserted between the promoter and the gene to enhance expression of the tetR gene. In absence of doxycycline, the tet repressor protein binds to the tet operators, blocking transcription. Induction by doxycycline releases the repression, permitting transcription of cre recombinase. Four founders were generated and individually analyzed for function and specificity. A representative line no. 1 was created. (J:157600)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gnrh1 (mouse) Summary of all recombinase alleles driven by Gnrh1. Comparative matrix view of recombinase activities Alliance mouse Gnrh1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:157600 Wolfe A, et al., Temporal and spatial regulation of CRE recombinase expression in gonadotrophin-releasing hormone neurones in the mouse. J Neuroendocrinol. 2008 Jul;20(7):909-16
• All: 1 reference(s)