Runx1^tm1.1Gss
Targeted Allele Detail

Summary

• Symbol: Runx1^tm1.1Gss
• Name: runt related transcription factor 1; targeted mutation 1.1, Gary S Stein
• MGI ID: MGI:4437264
• Synonyms: Runx1^Q307X
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm1.1Gss page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157660
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: Exon 8 was replaced with a floxed neo cassette and a modified exon 8 that contains a C to T transition producing a termination codon instead of glutamine at position 307 (Q307X), modeling genetic lesions observed in patients with leukemia and myeloproliferative disorders. Cre-mediated excision driven by the Tg(Prm-cre)70Og transgene carried by the parental PC3 ES cells removed the neo cassette upon transmission from male chimeras. (J:157660)

Expression

In Mice Carrying this Mutation:	36 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:157660 Dowdy CR, et al., Definitive hematopoiesis requires Runx1 C-terminal-mediated subnuclear targeting and transactivation. Hum Mol Genet. 2010 Mar 15;19(6):1048-57
• All: 1 reference(s)