Tgif2^tm1Dwot
Targeted Allele Detail

Summary

• Symbol: Tgif2^tm1Dwot
• Name: TGFB-induced factor homeobox 2; targeted mutation 1, David Wotton
• MGI ID: MGI:4437419
• Gene: Tgif2 Location: Chr2:156681927-156697490 bp, + strand Genetic Position: Chr2, 77.86 cM
• Alliance: Tgif2^tm1Dwot page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157256
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A GFP gene cassette was inserted 8 bp upstream of the start codon and a neo gene in reverse orientation was inserted 110 bp downstream of exon 2 resulting in the replacement of the coding region of exon 2 and part of intron 2. RT-PCR detected no transcript in homozygous mice and GFP was expressed. (J:157256)

Expression

In Mice Carrying this Mutation:

14 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tgif2 Mutation: 40 strains or lines available

References

• Original: J:157256 Powers SE, et al., Tgif1 and Tgif2 regulate Nodal signaling and are required for gastrulation. Development. 2010 Jan;137(2):249-59
• All: 6 reference(s)