Tg(Myh6*/tetO-Capn1)L2Gwd
Transgene Detail

Summary

• Symbol: Tg(Myh6*/tetO-Capn1)L2Gwd
• Name: transgene insertion L2, Gerald W Dorn II
• MGI ID: MGI:4437466
• Synonyms: Calpain 1 TG
• Transgene: Tg(Myh6*/tetO-Capn1)L2Gwd Location: unknown
• Alliance: Tg(Myh6*/tetO-Capn1)L2Gwd page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Myh6*/tetO-Capn1)L2Gwd expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Capn1 (MGI:88263)

• Mutation details: These MHCminTetO-Capn1 transgenic mice were generated by inserting the entire coding region of the mouse Calpain 1 gene into an MHCminTetO-promoter construct. The expression of this construct was driven by a mouse alpha-myosin heavy chain (alpha-MHC; Myh6) promoter under control of a tetracycline operator (tetO), followed by a human growth hormone polyadenylation (polyA) signal. This construct also contained 3 altered GATA sites and 2 altered thyroid-like response elements (TRE) maintaining low levels of cardiac-specific expression and decreased background. Three lines were created (L1, L2, and L3) with ascending levels of expression. (J:125131, J:133797)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:133797 Galvez AS, et al., Cardiomyocyte degeneration with calpain deficiency reveals a critical role in protein homeostasis. Circ Res. 2007 Apr 13;100(7):1071-8
• All: 5 reference(s)