Tg(Myh6*/tetO-Capn1)L3Gwd
Transgene Detail

Summary

• Symbol: Tg(Myh6*/tetO-Capn1)L3Gwd
• Name: transgene insertion L3, Gerald W Dorn II
• MGI ID: MGI:4437470
• Transgene: Tg(Myh6*/tetO-Capn1)L3Gwd Location: unknown
• Alliance: Tg(Myh6*/tetO-Capn1)L3Gwd page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: These MHCminTetO-Capn1 transgenic mice were generated by inserting the entire coding region of the mouse Calpain 1 gene into an MHCminTetO-promoter construct. The expression of this construct was driven by a mouse alpha-myosin heavy chain (alpha-MHC; Myh6) promoter under control of a tetracycline operator (tetO), followed by a human growth hormone polyadenylation (polyA) signal. This construct also contained 3 altered GATA sites and 2 altered thyroid-like response elements (TRE) maintaining low levels of cardiac-specific expression and decreased background. Three lines were created (L1, L2, and L3) with ascending levels of expression. (J:133797)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
tg1	Tg(Myh6*/tetO-Capn1)L3Gwd/0	involves: FVB/N

Phenotypes:

Affected Systems	tg1
show or hide all annotated terms
cardiovascular system	√
myocardium necrosis	√
increased heart weight	√
abnormal heart left ventricle morphology	√
dilated heart ventricle	√
decreased heart ventricle muscle contractility	√
congestive heart failure	√
heart inflammation	√
growth/size/body	√
increased heart weight	√
immune system	√
heart inflammation	√
mortality/aging	√
premature death	√
muscle	√
myocardium necrosis	√
decreased heart ventricle muscle contractility	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:133797 Galvez AS, et al., Cardiomyocyte degeneration with calpain deficiency reveals a critical role in protein homeostasis. Circ Res. 2007 Apr 13;100(7):1071-8
• All: 1 reference(s)