Tg(SFTPC-rtTA,tetO-Tnf)320-1Gwho
Transgene Detail

Summary

• Symbol: Tg(SFTPC-rtTA,tetO-Tnf)320-1Gwho
• Name: transgene insertion 320-1, Gary W Hoyle
• MGI ID: MGI:4437863
• Synonyms: Tg(SFTPC-rtTA,TRE-Tnf)320-1Gwho, TRE TNF-a
• Transgene: Tg(SFTPC-rtTA,tetO-Tnf)320-1Gwho Location: unknown
• Alliance: Tg(SFTPC-rtTA,tetO-Tnf)320-1Gwho page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(SFTPC-rtTA,tetO-Tnf)320-1Gwho expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tnf (MGI:104798)

• Mutation details: A transgenic construct containing the rtTA gene under the control of the human lung-specific SFTPC promoter and intronic and polyadenylation sequences from the human GH gene, and another transgenic construct containing the murine Tnf gene under the control of TRE (tetO), were co-injected into fertilized mouse eggs to generate transgenic mice in which the two constructs were integrated at the same chromosomal location. Line 320-1 was extensively investigated. Northern blot analysis showed that Tnf mRNA levels were significantly increased in the lung, and no Tnf transgene message was observed in the heart, liver, kidney, muscle, spleen, or thymus; however, a weak band was observed in the brain after doxycycline treatment. TNF activity was significantly increased about 6-fold in the lungs of doxycycline-treated transgenic mice. (J:97297)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:97297 Vuillemenot BR, et al., Lymphoid tissue and emphysema in the lungs of transgenic mice inducibly expressing tumor necrosis factor-alpha. Am J Respir Cell Mol Biol. 2004 Apr;30(4):438-48
• All: 3 reference(s)