Gnb1^{Gt(prvSStrap)4B8Yiw}
Gene trapped Allele Detail

Summary

• Symbol: Gnb1^{Gt(prvSStrap)4B8Yiw}
• Name: guanine nucleotide binding protein (G protein), beta 1; gene trap 4B8, Yoichiro Iwakura
• MGI ID: MGI:4438390
• Synonyms: Gbeta1^-
• Gene: Gnb1 Location: Chr4:155575818-155643726 bp, + strand Genetic Position: Chr4, 86.17 cM
• Alliance: Gnb1^{Gt(prvSStrap)4B8Yiw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157907
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A gene trap vector, containing the splice acceptor site, beta-gal, and hygromycin phosphotransferase gene (hygR), was randomly inserted into the first intron of the gene, creating the 4B8 mutant ES cell line. RT-PCR analyses indicated that RNA transcripts were not detected in E10.5 mutant embryos. Western blot analysis showed that the protein was absent in mutant retina. (J:157907)

Expression

In Mice Carrying this Mutation:	29 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gnb1 Mutation: 62 strains or lines available

References

• Original: J:157907 Okae H, et al., Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice. Dev Dyn. 2010 Apr;239(4):1089-101
• All: 4 reference(s)