Slc20a1^tm1Lbek
Targeted Allele Detail

Summary

• Symbol: Slc20a1^tm1Lbek
• Name: solute carrier family 20, member 1; targeted mutation 1, Laurent Beck
• MGI ID: MGI:4438612
• Synonyms: PiT1^neo
• Gene: Slc20a1 Location: Chr2:129040684-129053536 bp, + strand Genetic Position: Chr2, 62.79 cM
• Alliance: Slc20a1^tm1Lbek page

Small size, pale coloration and abnormal red blood cell morphology in Slc20a1^tm1Lbek/Slc20a1^tm1Lbek mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157997
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

Allele Type: Targeted (Hypomorph) Mutation: Insertion Mutation details: An FRT flanked neo cassette was inserted in intron 5 and exon 5 was flanked 5' and 3' with loxP sites 532 bp upstream and 934 bp downstream. Aberrant splicing due to the use of cryptic splice sites results in a hybrid mRNA containing an additional portion of intron 5 and the coding sequence of neo. Wild-type RNA is reduced in this hypomorphic allele. (J:157997)

Generation of the Slc20a1^tm1Lbek, Slc20a1^tm1.1Lbek and Slc20a1^tm1.2Lbek alleles

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc20a1 Mutation: 36 strains or lines available

References

• Original: J:157997 Beck L, et al., The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development. PLoS One. 2010;5(2):e9148
• All: 3 reference(s)