Dck^tm1.1Radu
Targeted Allele Detail

Summary

• Symbol: Dck^tm1.1Radu
• Name: deoxycytidine kinase; targeted mutation 1.1, Caius G Radu
• MGI ID: MGI:4440453
• Gene: Dck Location: Chr5:88912872-88931136 bp, + strand Genetic Position: Chr5, 43.96 cM, cytoband E2
• Alliance: Dck^tm1.1Radu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158373
• Parent Cell Line: LW1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 was floxed and an FRT flanked neo cassette was inserted within intron 3 by homologous recombination. Exon 3 is deleted by cre mediated recombination which eliminated the catalytic domain and also caused a frameshift mutation and early termination of the protein. Loss of function was confirmed by in vitro kinase assay as well as PET and CT imaging with labeled probes. (J:158373)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dck Mutation: 20 strains or lines available

References

• Original: J:158373 Toy G, et al., Requirement for deoxycytidine kinase in T and B lymphocyte development. Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5551-6
• All: 2 reference(s)