Bid^tm1.1Ast
Targeted Allele Detail

Summary

• Symbol: Bid^tm1.1Ast
• Name: BH3 interacting domain death agonist; targeted mutation 1.1, Andreas Strasser
• MGI ID: MGI:4440571
• Synonyms: Bid^-
• Gene: Bid Location: Chr6:120870080-120894074 bp, - strand Genetic Position: Chr6, 57.02 cM
• Alliance: Bid^tm1.1Ast page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158191
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The allele was created by crossing heterozygous Bid^tm1Ast mice with Tg(CMV-cre)1Cgn cre deleter mice to remove neo cassette, leaving a single loxP site and deleting approximately 4.5 kb region of the bid locus containing exons 1 and 2 plus part of exon 3, including the crucial BH3 domain. Western blot analysis on spleen and thymus samples and thymocytes derived from homozygous mice demonstrated that no detectable protein is produced from this allele. (J:158191)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bid Mutation: 44 strains or lines available

References

• Original: J:158191 Kaufmann T, et al., The BH3-only protein bid is dispensable for DNA damage- and replicative stress-induced apoptosis or cell-cycle arrest. Cell. 2007 Apr 20;129(2):423-33
• All: 15 reference(s)