Ptgs2^tm2.1Hahe
Targeted Allele Detail

Summary

• Symbol: Ptgs2^tm2.1Hahe
• Name: prostaglandin-endoperoxide synthase 2; targeted mutation 2.1, Harvey R Herschman
• MGI ID: MGI:4440835
• Synonyms: Cox2^fluc, Cox-2^luc, Cox2^luc
• Gene: Ptgs2 Location: Chr1:149975851-149983978 bp, + strand Genetic Position: Chr1, 63.84 cM, cytoband H1
• Alliance: Ptgs2^tm2.1Hahe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158934
• Parent Cell Line: LW1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A luciferase coding sequence with an SV40 3' UTR and a floxed neo cassette was inserted downstream of the translation initiation site, replacing the fisrt 4 coding exons. Cre-mediated recombination removed the neo cassette. The absence of protein expression was confirmed by western blot analysis on embryonic fibroblast extracts. (J:158934)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ptgs2 Mutation: 73 strains or lines available

Notes

This allele is not sensitive to knockdown by Col1a1^{tm1(tetO-RNAi:Ptgs2;GFP)Hahe}.

References

• Original: J:158934 Ishikawa TO, et al., Imaging cyclooxygenase-2 (Cox-2) gene expression in living animals with a luciferase knock-in reporter gene. Mol Imaging Biol. 2006 May-Jun;8(3):171-87
• All: 9 reference(s)