Rab25^tm1Jrgo
Targeted Allele Detail

Summary

• Symbol: Rab25^tm1Jrgo
• Name: RAB25, member RAS oncogene family; targeted mutation 1, James R Goldenring
• MGI ID: MGI:4440861
• Synonyms: Rab25^-
• Gene: Rab25 Location: Chr3:88449335-88455586 bp, - strand Genetic Position: Chr3, 38.87 cM, cytoband E3-F1
• Alliance: Rab25^tm1Jrgo page

Rab25^tm1Jrgo/Rab25^tm1Jrgo Apc^Min/Apc⁺ mice exhibit increased intestinal adenoma formation compared to Apc^Min/Apc⁺ mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158733
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutation: Insertion Mutation details: The neomycin resistance gene along with 3 tandem in-frame stop codons was inserted into the second exon. Western blot and immunostaining indicated a lack of protein expression in the intestines of homozygous mice. (J:158733)

Construction of Rab25^tm1Jrgo allele

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rab25 Mutation: 18 strains or lines available

References

• Original: J:158733 Nam KT, et al., Loss of Rab25 promotes the development of intestinal neoplasia in mice and is associated with human colorectal adenocarcinomas. J Clin Invest. 2010 Mar;120(3):840-9
• All: 2 reference(s)