Pag1^tm1.1Tara
Targeted Allele Detail

Summary

• Symbol: Pag1^tm1.1Tara
• Name: phosphoprotein associated with glycosphingolipid microdomains 1; targeted mutation 1.1, Alexander Tarakhovsky
• MGI ID: MGI:4441057
• Synonyms: Cbp^-
• Gene: Pag1 Location: Chr3:9752539-9898739 bp, - strand Genetic Position: Chr3, 2.47 cM
• Alliance: Pag1^tm1.1Tara page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159011
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette was inserted in intron 1 and a third loxP site was placed in intron 2 just downstream of exon 2. The selection cassette was then removed from properly targeted ES cells by transient cre expression. Homozygous mice were crossed to Tg(EIIa-cre)C5379Lmgd mice to delete exon 2. Western blot of protein from thymocytes and splenocytes of mice homozygous for the exon 2 deletion lacked expression of native protein and trucated forms. (J:159011)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pag1 Mutation: 21 strains or lines available

References

• Original: J:159011 Dobenecker MW, et al., The ubiquitously expressed Csk adaptor protein Cbp is dispensable for embryogenesis and T-cell development and function. Mol Cell Biol. 2005 Dec;25(23):10533-42
• All: 1 reference(s)