Prox1^{tm5(GFP/cre)Gco}
Targeted Allele Detail

Summary

• Symbol: Prox1^{tm5(GFP/cre)Gco}
• Name: prospero homeobox 1; targeted mutation 5, Guillermo Oliver
• MGI ID: MGI:4441289
• Synonyms: Prox1^Cre, Prox1^GFPCre
• Gene: Prox1 Location: Chr1:189850232-189902911 bp, - strand Genetic Position: Chr1, 95.85 cM
• Alliance: Prox1^{tm5(GFP/cre)Gco} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158959
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutations: Insertion, Intragenic deletion
• Prox1^{tm5(GFP/cre)Gco} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prox1 (MGI:97772)

• Mutation details: A GFPCre expression cassette was inserted into the transcription start site (ATG) of the Prox1 genomic locus along with a neomycin selection cassette. A thymidine kinase negative-selection cassette was also included. The targeting construct was electroporated into W9.5 mouse ES cells. Following selection and screening via standard methods, correctly targeted ES cell clones were used to generate the chimeric mice. (J:158959)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prox1 (mouse) Summary of all recombinase alleles driven by Prox1. Alliance mouse Prox1 gene page

Expression

In Mice Carrying this Mutation:	68 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prox1 Mutation: 43 strains or lines available

References

• Original: J:158959 Srinivasan RS, et al., The nuclear hormone receptor Coup-TFII is required for the initiation and early maintenance of Prox1 expression in lymphatic endothelial cells. Genes Dev. 2010 Apr 1;24(7):696-707
• All: 16 reference(s)