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Flt4tm1.1Ichi
Targeted Allele Detail
Summary
Symbol: Flt4tm1.1Ichi
Name: FMS-like tyrosine kinase 4; targeted mutation 1.1, Hirotake Ichise
MGI ID: MGI:4441407
Synonyms: Vegfr3-EGFP
Gene: Flt4  Location: Chr11:49500506-49543566 bp, + strand  Genetic Position: Chr11, 29.69 cM, cytoband A5-B1
Alliance: Flt4tm1.1Ichi page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:159024
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  (C57BL/6J x 129S/SvEv)F1
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA vector composed a 5' fragment of Flt4, an EGFP cDNA with a Pgk polyA signal sequence followed by an FRT-flanked Pgk-gb2-neo cassette ligated upstream of the first ATG of the Flt4 gene, and a 3' homology arm encoding an intronic region between exons 1 and 2 of Flt4 was used for targeting the Flt4 locus in (C57BL/6J x 129/SvEv)F1 ES cells. Exon 1 was replaced by EGFP after homologous recombination. The neo cassette was deleted in heterozygous mice by crossing with FLP66 deleter mice. No Flt4 protein is detected by Western blot in homozygous embryos. (J:159024)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Flt4 Mutation:  77 strains or lines available
References
Original:  J:159024 Ichise T, et al., H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice. Development. 2010 Mar;137(6):1003-13
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory