Myo1e^tm1.1Flv
Targeted Allele Detail

Summary

• Symbol: Myo1e^tm1.1Flv
• Name: myosin IE; targeted mutation 1.1, Richard A Flavell
• MGI ID: MGI:4441413
• Synonyms: Myo1e^-
• Gene: Myo1e Location: Chr9:70114632-70307048 bp, + strand Genetic Position: Chr9, 39.41 cM, cytoband D
• Alliance: Myo1e^tm1.1Flv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159163
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP-flanked neomycin resistance cassette was introduced to intron 4 and an additional loxP site was placed in intron 3. Cre-mediated recombination resulted in germline deletion of exon 4 and the neomycin cassette. The absence of protein expression was confirmed by western blot analysis on kidney and spleen extracts. (J:159163)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myo1e Mutation: 91 strains or lines available

References

• Original: J:159163 Krendel M, et al., Disruption of Myosin 1e promotes podocyte injury. J Am Soc Nephrol. 2009 Jan;20(1):86-94
• All: 8 reference(s)