Actn4^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Actn4^{tm1a(EUCOMM)Wtsi}
• Name: actinin alpha 4; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4441842
• Gene: Actn4 Location: Chr7:28592673-28661765 bp, - strand Genetic Position: Chr7, 16.94 cM
• Alliance: Actn4^{tm1a(EUCOMM)Wtsi} page
• IMPC: Actn4 gene page

Mutation origin

• Mutant Cell Lines: EPD0525_2_A10, EPD0525_2_A12, EPD0525_2_B09, EPD0525_2_B11, EPD0525_2_B12, EPD0525_2_C10, EPD0525_2_C11, EPD0525_2_C12, EPD0525_2_D09, EPD0525_2_D10, EPD0525_2_D11, EPD0525_2_D12, EPD0525_2_E09, EPD0525_2_E10, EPD0525_2_E11, EPD0525_2_E12, EPD0525_2_F10, EPD0525_2_F11, EPD0525_2_F12, EPD0525_2_G09, EPD0525_2_G10, EPD0525_2_G11, EPD0525_2_H09
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 28614391 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 28615171. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Structures Affected by this Mutation:

21 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Actn4 Mutation: 137 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 8 reference(s)