Sox18^{tm1(GFP/cre/ERT2)Pzg}
Targeted Allele Detail

Summary

• Symbol: Sox18^{tm1(GFP/cre/ERT2)Pzg}
• Name: SRY (sex determining region Y)-box 18; targeted mutation 1, Pumin Zhang
• MGI ID: MGI:4442184
• Synonyms: Sox18-GCE, Sox18-GFP-Cre-ERT2, Sox18^{tm1(cre/ERT2)Zhang}
• Gene: Sox18 Location: Chr2:181311630-181313433 bp, - strand Genetic Position: Chr2, 103.71 cM
• Alliance: Sox18^{tm1(GFP/cre/ERT2)Pzg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159099
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Sox18^{tm1(GFP/cre/ERT2)Pzg} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sox18 (MGI:103559)

• Mutation details: Mice with this mutation have a tamoxifen-inducible Cre-mediated recombination system and, independently, express green fluorescent protein (GFP) from the promoter of the endogenous gene. A GFP-Cre/ERT2 cassette (GCE) with a polyadenylation signal followed by a frt site-flanked kanamycin and neomycin resistance cassette was inserted in the first targeting exon of the endogenous gene immediately following the ATG translation initiation codon. Although no green fluorescence is visible in whole heterozygous mutant E15.5 embryos or their urogenital tracts, immunohistochemical analysis detects GFP protein in kidneys and ovaries of these embryos. Following four-day tamoxifen induction of Cre recombinase activity in E15.5 embryos bearing this allele and a Cre recombinase-inducible lacZ reporter gene, beta-galactosidase activity is detected weakly by X-gal staining and beta-galactosidase protein more strongly by immunohistochemical analysis in the kidneys and ovaries. (J:159099)
• Inheritance: Not Specified

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sox18 (mouse) Summary of all recombinase alleles driven by Sox18. Alliance mouse Sox18 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sox18 Mutation: 26 strains or lines available

References

• Original: J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010
• All: 6 reference(s)