Chrna3^tm1.1Hwrt
Targeted Allele Detail

Summary

• Symbol: Chrna3^tm1.1Hwrt
• Name: cholinergic receptor, nicotinic, alpha polypeptide 3; targeted mutation 1.1, Edward Hawrot
• MGI ID: MGI:4442209
• Synonyms: alpha3[5] nAChR
• Gene: Chrna3 Location: Chr9:54917401-54933846 bp, - strand Genetic Position: Chr9, 29.84 cM
• Alliance: Chrna3^tm1.1Hwrt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157611
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting construct was made containing a mutated exon 5 of mouse nicotinic acetylcholine receptor (nAChR) neuronal alpha3 subunit (alpha3[5]), and a loxP-flanked neomycin (neo) resistance cassette upstream of exon 5. This mutated alpha3[5] gene contained five altered nucleotides, Y184W, E187W, I188V, K189Y, and N191T, in the loop C region corresponding to residues from the alpha1 subunit of nAchR from Torpedo californica, the Pacific electric ray which conveys alpha-bungarotoxin (alphaBTX) sensitivity. Cre mediated recombination removed the neo cassette. (J:157611)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrna3 Mutation: 35 strains or lines available

References

• Original: J:157611 Caffery PM, et al., Engineering neuronal nicotinic acetylcholine receptors with functional sensitivity to alpha-bungarotoxin: a novel alpha3-knock-in mouse. Eur J Neurosci. 2009 Dec 3;30(11):2064-76
• All: 2 reference(s)