Tg(CAG-Bgeo,-tsA58T)T26Ichi
Transgene Detail

Summary

• Symbol: Tg(CAG-Bgeo,-tsA58T)T26Ichi
• Name: transgene insertion T26, Hirotake Ichise
• MGI ID: MGI:4442215
• Synonyms: CAG-Betageo-TsA58T tg, LBSL-tsTAg, T26 Tg
• Transgene: Tg(CAG-Bgeo,-tsA58T)T26Ichi Location: unknown
• Alliance: Tg(CAG-Bgeo,-tsA58T)T26Ichi page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:159206
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene construct has a loxP-flanked Beta-geo gene driven by the CAG promoter, followed by the SV40 tsA58 large T antigen (cDNA for the SV40 TAg and the 3' portion of tsA58T antigen sequence with the A438V mutation.) In the absence of cre-mediated recombination, Beta-geo is expressed and detected by X-gal staining. Removal of the loxP-flanked Beta-geo results in expression of the tsA58T antigen, leading to temperature-dependent cell-type independent cell proliferation, (J:159206)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:159206 Yamaguchi T, et al., Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse. FEBS J. 2008 May;275(9):1988-98
• All: 5 reference(s)