Pax3^tm2Joe
Targeted Allele Detail

Summary

• Symbol: Pax3^tm2Joe
• Name: paired box 3; targeted mutation 2, Jonathan A Epstein
• MGI ID: MGI:4442462
• Synonyms: Pax3^NCE-neo
• Gene: Pax3 Location: Chr1:78077904-78173771 bp, - strand Genetic Position: Chr1, 39.79 cM
• Alliance: Pax3^tm2Joe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159124
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Modified regulatory region, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 674 bp neural crest enhancer region within the upstream genomic region was replaced by a flowed PGK-neo cassette. Immunohistochemistry showed a drastic reduction or complete loss of protein expression in homozygous embryos at E12.5. Removal of the PGK-neo cassette with Cre-expressing mice allows for tissue-specific rescue. (J:159124)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax3 Mutation: 50 strains or lines available

References

• Original: J:159124 Degenhardt KR, et al., Distinct enhancers at the Pax3 locus can function redundantly to regulate neural tube and neural crest expressions. Dev Biol. 2010 Mar 15;339(2):519-27
• All: 1 reference(s)