Trp73^tm2Mak
Targeted Allele Detail

Summary

• Symbol: Trp73^tm2Mak
• Name: transformation related protein 73; targeted mutation 2, Tak Mak
• MGI ID: MGI:4442798
• Synonyms: deltaNp73^-
• Gene: Trp73 Location: Chr4:154140706-154224332 bp, - strand Genetic Position: Chr4, 83.79 cM
• Alliance: Trp73^tm2Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157905
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s), Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3' was replaced with a neo cassette by homologous recombination. This targets the delta Np73 isoforms but not TAp73 isoforms. The loss of Np73 isoform mRNA expression was confirmed by quantitative RT-PCR in MEFs and western blot analysis of lung tissue confirmed loss of isoform specific protein expression. Quantitative RT-PCR of liver, testis, and lung confirmed the retention of TAp73 isoform mRNA expression. (J:157905)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Trp73 Mutation: 46 strains or lines available

References

• Original: J:157905 Wilhelm MT, et al., Isoform-specific p73 knockout mice reveal a novel role for {Delta}Np73 in the DNA damage response pathway. Genes Dev. 2010 Mar 15;24(6):549-60
• All: 3 reference(s)