Pclo^tm3.2Sud
Targeted Allele Detail

Summary

• Symbol: Pclo^tm3.2Sud
• Name: piccolo (presynaptic cytomatrix protein); targeted mutation 3.2, Thomas C Sudhof
• MGI ID: MGI:4442914
• Synonyms: Pic^KO
• Gene: Pclo Location: Chr5:14564932-14913473 bp, + strand Genetic Position: Chr5, 6.27 cM, cytoband A2
• Alliance: Pclo^tm3.2Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159311
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cre mediated recombination removed the modified exon 14. The frt flanked neo cassette remained intact. The absence of protein expression was confirmed by western blot analysis on brain extracts. (J:159311)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pclo Mutation: 267 strains or lines available

References

• Original: J:159311 Mukherjee K, et al., Piccolo and bassoon maintain synaptic vesicle clustering without directly participating in vesicle exocytosis. Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6504-9
• All: 1 reference(s)