Mfn2^tm1.1Balo
Targeted Allele Detail

Summary

• Symbol: Mfn2^tm1.1Balo
• Name: mitofusin 2; targeted mutation 1.1, Robert Balo
• MGI ID: MGI:4442975
• Synonyms: Mfn2^H361Y
• Gene: Mfn2 Location: Chr4:147958056-147989161 bp, - strand Genetic Position: Chr4, 78.56 cM
• Alliance: Mfn2^tm1.1Balo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159219
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A floxed HA-tagged human cDNA with nucleotide substitutions that result in the amino acid substitution of tyrosine for histidine at position 361 (H361Y), GFP, and an frt flanked neo cassette were insered into exon 4, which contains the translation initiation site. Flp mediated recombination removed the neo cassette. The absence of protein expression was confirmed by western blot analysis on E12.5 embryo extracts. (J:159219)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mfn2 Mutation: 27 strains or lines available

References

• Original: J:159219 Misko A, et al., Mitofusin 2 is necessary for transport of axonal mitochondria and interacts with the Miro/Milton complex. J Neurosci. 2010 Mar 24;30(12):4232-40
• All: 1 reference(s)