Mirc32^tm1Gcon
Targeted Allele Detail

Summary

• Symbol: Mirc32^tm1Gcon
• Name: microRNA cluster 32, including Mir143 and Mir145a; targeted mutation 1, Gianluigi Condorelli
• MGI ID: MGI:4443000
• Synonyms: Mir145/Mir143^tm1Gcon
• Gene: Mirc32 Location: Chr18:61780896-61782329 bp Genetic Position: Chr18, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159593
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The exon specifying Mir143 was replaced with a lacZ gene and floxed neo cassette. Mir143 and Mir145 are a part of the same transcriptional unit. Northern blot and qRT-PCR confirmed the absence of Mir143 and Mir145 transcripts in aorta extracts. (J:159593)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc32 Mutation: 3 strains or lines available

References

• Original: J:159593 Elia L, et al., The knockout of miR-143 and -145 alters smooth muscle cell maintenance and vascular homeostasis in mice: correlates with human disease. Cell Death Differ. 2009 Dec;16(12):1590-8
• All: 4 reference(s)