Med1^tm2Rgr
Targeted Allele Detail

Summary

• Symbol: Med1^tm2Rgr
• Name: mediator complex subunit 1; targeted mutation 2, Robert G Roeder
• MGI ID: MGI:4443002
• Synonyms: MED1^KI
• Gene: Med1 Location: Chr11:98042980-98084119 bp, - strand Genetic Position: Chr11, 61.75 cM, cytoband D
• Alliance: Med1^tm2Rgr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159303
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: Exons 5 through 8 were replaced with a cDNA in which nucleotide substitutions resulted in the amino acid substitution of alanine for lysine within the LxxLL motif (to LxxAA). A floxed neo cassette was inserted for selection purposes. Normal levels of protein expression were confirmed by western blot analysis. (J:159303)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Med1 Mutation: 83 strains or lines available

References

• Original: J:159303 Jiang P, et al., Key roles for MED1 LxxLL motifs in pubertal mammary gland development and luminal-cell differentiation. Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6765-70
• All: 3 reference(s)