Dok3^tm1Ppp
Targeted Allele Detail

Summary

• Symbol: Dok3^tm1Ppp
• Name: docking protein 3; targeted mutation 1, Pier Paolo Pandolfi
• MGI ID: MGI:4443003
• Gene: Dok3 Location: Chr13:55671044-55677109 bp, - strand Genetic Position: Chr13, 30.06 cM
• Alliance: Dok3^tm1Ppp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158011
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exons 3-5 were disrupted by insertion of a cassette containing GFP (in frame) and neo (in reverse orientation). No GFP expression was detected in lung or peripheral blood. The absence of protein expression was confirmed by western blot analysis on splenocyte extracts. (J:158011)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Dok3^tm1Ppp/Dok3^tm1Ppp	129S1/Sv-Dok3^tm1Ppp
cx2	Dok1^tm1Ppp/Dok1^tm1Ppp Dok3^tm1Ppp/Dok3^tm1Ppp	129S1/Sv-Dok1^tm1Ppp Dok3^tm1Ppp
cx3	Dok1^tm1Ppp/Dok1^tm1Ppp Dok2^tm1Ppp/Dok2^tm1Ppp Dok3^tm1Ppp/Dok3^tm1Ppp	129S1/Sv-Dok1^tm1Ppp Dok2^tm1Ppp Dok3^tm1Ppp

Phenotypes:

Affected Systems	hm1	cx2	cx3
show or hide all annotated terms
growth/size/body			√
pulmonary hyperplasia			√
hematopoietic system			√
increased leukocyte cell number			√
immune system			√
increased leukocyte cell number			√
mortality/aging			√
premature death			√
neoplasm	√	√	√
increased chronic myelocytic leukemia incidence			√
increased lung tumor incidence			√
increased lung adenocarcinoma incidence	√	√	√
respiratory system	√	√	√
pulmonary hyperplasia			√
increased lung tumor incidence			√
increased lung adenocarcinoma incidence	√	√	√
increased bronchioalveolar stem cell number			√
abnormal pulmonary alveolus morphology			√
increased type II pneumocyte number			√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Dok3^tm1Ppp

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dok3 Mutation: 18 strains or lines available

References

• Original: J:158011 Berger AH, et al., Identification of DOK genes as lung tumor suppressors. Nat Genet. 2010 Mar;42(3):216-23
• All: 3 reference(s)