Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Ruslan Medzhltov
• MGI ID: MGI:4443069
• Synonyms: R26-mp53
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158953
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Dominant negative, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Trp53 (MGI:98834)

• Mutation details: The inserted construct contains a "CAG promoter," which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene, driving ubiquitous expression of the mouse cDNA with nucleotide substitutions that results in the amino acid substitution of histidine for arginine at position 172 (R172H). The construct is preceded by a floxed STOP cassette and followed by an IRES-EGFP. The R172H mutation is predicted to create a dominant-negative product that is only expressed following cre mediated recombination to remove the STOP cassette. (J:158953)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:158953 Bondar T, et al., p53-mediated hematopoietic stem and progenitor cell competition. Cell Stem Cell. 2010 Apr 2;6(4):309-22
• All: 2 reference(s)