Gt(ROSA)26Sor^{tm1.1(rtTA,tetO-cre)Bkmn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(rtTA,tetO-cre)Bkmn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Cristina M Backman
• MGI ID: MGI:4443293
• Synonyms: R26^rtTA-TRECre
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(rtTA,tetO-cre)Bkmn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159515
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(rtTA,tetO-cre)Bkmn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  bacteria	tetO

• Mutation details: A targeting vector was generated that contained 5 + 3' homology arms to the first intron of the Gt(ROSA)26Sor locus, a cassette containing a splice acceptor, the improved rtTA-M2 element followed by a human TP53 intron and in opposite orientation, the tet-response element TRE-tight promoter driving a cre coding sequence (tetO-cre). The vector also contained an FRT-flanked neo selection cassette. Homologous recombination was done in R1 ES cells. Germline mice were produced and subsequently crossed to Flpe deleter mice to delete the neo cassette. (J:159515)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	tetO (bacteria) Summary of all recombinase alleles driven by tetO.

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:159515 Backman CM, et al., Generalized tetracycline induced Cre recombinase expression through the ROSA26 locus of recombinant mice. J Neurosci Methods. 2009 Jan 15;176(1):16-23
• All: 2 reference(s)