Tra2b^tm1.2Bwir
Targeted Allele Detail

Summary

• Symbol: Tra2b^tm1.2Bwir
• Name: transformer 2 beta; targeted mutation 1.2, Brunhilde Wirth
• MGI ID: MGI:4450921
• Synonyms: Sfrs10^-
• Gene: Tra2b Location: Chr16:22063302-22084755 bp, - strand Genetic Position: Chr16, 13.18 cM
• Alliance: Tra2b^tm1.2Bwir page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159448
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single loxP site and an frt flanked neomycin resistance cassette were inserted upstream of exon 4 and a second loxP site was inserted downstream of the exon. Exon 4 and the neomycin resistance cassette were excised in mice by crossing to the cre deleter line, Tg(CMV-cre)1Cgn. Deletion of exon 4 results in a frameshift and a premature stop codon in exon 5. (J:159448)

Expression

In Mice Carrying this Mutation:	10 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tra2b Mutation: 22 strains or lines available

References

• Original: J:159448 Mende Y, et al., Deficiency of the splicing factor Sfrs10 results in early embryonic lethality in mice and has no impact on full-length SMN/Smn splicing. Hum Mol Genet. 2010 Jun 1;19(11):2154-67
• All: 1 reference(s)