Acacb^tm1.1Lowl
Targeted Allele Detail

Summary

• Symbol: Acacb^tm1.1Lowl
• Name: acetyl-Coenzyme A carboxylase beta; targeted mutation 1.1, Bradford B Lowell
• MGI ID: MGI:4450932
• Synonyms: Acc2^flox
• Gene: Acacb Location: Chr5:114284748-114388822 bp, + strand Genetic Position: Chr5, 55.97 cM
• Alliance: Acacb^tm1.1Lowl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159285
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites were positioned upstream of the preceding exon and downstream of the biotin binding domain exon, with an Frt flanked kanamycin resistance cassette inserted before the 3' loxP site. The Frt-flanked selection cassette was removed by Flpe recombinase-mediated recombination. Splicing of the remaining exons following Cre-mediated deletion would introduce a frameshift and nonsense mutation after Asp865 in any translated protein. (J:159285)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Acacb Mutation: 109 strains or lines available

References

• Original: J:159285 Olson DP, et al., Gene knockout of Acc2 has little effect on body weight, fat mass, or food intake. Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7598-603
• All: 8 reference(s)